منابع مشابه
Crossed immunoelectrophoresis of human platelet membranes.
Human platelet membranes were prepared by the glycerol-lysis technique of Barber and Jamieson (Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem 245, 6357-6365), solubilized in 1% Triton X-100 and subjected to crossed immunoelectrophoresis, in 1% agarose, employing a rabbit anti-human platelet membrane antibody in the second dimension. Approximately 20 immunoprecipitates (numbered 1 to ...
متن کاملCharacterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.
Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four st...
متن کاملImmunoelectrophoresis versus Electropho- Resis in Study of Dysgammaglobolinemia
The total 476 sera were examined by a routine electrophoresis and further by the use of immunoelectrophoresis. 1) Among the 42 broad banded hypergammaglobulinemics, neither M-components nor a lack of a.ny class of immunoglobulin was detected. 2 ) Out of the 23 hypogammaglobulinemics, 3 cases of dysgammaglobulinemia were found only after the immunoelectrophoresis. 3 ) All of the 51 cases with a ...
متن کاملRapid two-dimensional immunoelectrophoresis of human serum proteins.
We describe two versions of a two-dimensional immunoelectrophoretic system and illustrate their use for separating and estimating human serum proteins. Each is sensitive, rapid, and simple to perform, and requires no special apparatus and only 0.1-0.6 ml of antiserum. Both procedures appear to be potentially useful in the clinical laboratory. Time required to obtain a stained pattern varies fro...
متن کاملRocket and Two Dimensional Immunoelectrophoresis in Diagnosis of Caprine Brucellosis
BACKGROUND Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. METHODS Sera from 15 sheep, without any histo...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1982
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456328201900210